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Antibody used in IHC on Human Liver.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in IHC on Human Liver.

IKZF3 Antibody

CATALOG NUMBER: 28-994

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IKZF3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
IKZF3 antibody can be used for detection of IKZF3 by ELISA at 1:312500. IKZF3 antibody can be used for detection of IKZF3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
52 kDa, 58 kDa, 53 kDa, 54 kDa, 47 kDa, 49 kDa
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store IKZF3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
IKZF3
Additional Names:
IKZF3, AIO, AIOLOS, ZNFN1A3, RP11-94L15.2
Protein Accession Number:
NP_899051
Protein Gi Number:
38045959
Ncbi Gene Id Number:
22806
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IKZF3 is a member of the Ikaros family of zinc-finger proteins. It is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers.This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoetic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described.
Background Reference 1:
Koipally, J. (2002) J. Biol. Chem. 277 (31), 27697-27705.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-994

List Size:
100 ul

List Price:

$519.00

Datasheet
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