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Western blot analysis of Recombinant Mouse IL-1 alpha Protein using IL-1 alpha Rabbit mAb at 1:10000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 10 ng per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180 s.
Western blot analysis of lysates from RAW 264.7 cells using IL-1 alpha Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. RAW 264.7 cells were treated with LPS (1 μg/mL) for 8 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5 s.
Western blot analysis of Recombinant Mouse IL-1 alpha Protein using IL-1 alpha Rabbit mAb at 1:10000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 10 ng per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180 s.

IL-1 alpha Rabbit Recombinant Antibody (82-398)

CATALOG NUMBER: 82-398

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:1000 - 1:10000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Recombinant Mouse IL-1 alpha Protein,RAW 264.7 treated with LPS
Predicted Molecular Weight:
31kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Il1a
Additional Names:
Il-1a; IL-1 alpha
Protein Accession Number:
P01582
Ncbi Gene Id Number:
16175
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables cytokine activity and interleukin-1 receptor binding activity. Involved in positive regulation of angiogenesis and positive regulation of vascular endothelial growth factor production. Acts upstream of or within several processes, including cell surface receptor signaling pathway; connective tissue replacement involved in inflammatory response wound healing; and positive regulation of cytokine production. Located in cytoplasm and extracellular space. Is expressed in several structures, including alimentary system; brain; hemolymphoid system gland; reproductive system; and respiratory system. Human ortholog(s) of this gene implicated in several diseases, including Fabry disease; Schnitzler syndrome; autoimmune disease (multiple); hematologic cancer (multiple); and lung disease (multiple). Orthologous to human IL1A (interleukin 1 alpha).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-398

List Size:
100 uL

List Price:

$477.00

Datasheet
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