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In a sandwich ELISA (assuming 100μl/well), a concentration of 0.25-0.50 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human IL-1 beta when used with ProSci's Biotinylated Antigen Affinity Purified Anti-Human IL-1 beta as the detection antibody at a concentration of approximately 0.25-0.50 μg/ml
To detect Human IL-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-1 beta is 2.0-4.0 ng/lane, under reducing conditions and 4.0-8.0 ng/lane, under non-reducing conditions.
In a sandwich ELISA (assuming 100μl/well), a concentration of 0.25-0.50 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human IL-1 beta when used with ProSci's Biotinylated Antigen Affinity Purified Anti-Human IL-1 beta as the detection antibody at a concentration of approximately 0.25-0.50 μg/ml

IL-1 beta Antibody

CATALOG NUMBER: 38-306

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Produced in BALB/c mice immunized with highly pure Recombinant Human IL-1beta. Anti-Human IL-1 beta-specific antibody was purified from cell culture by Protein G affinity chromatography.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 0.25-0.50 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human IL-1 beta when used with ProSci’s Biotinylated Antigen Affinity Purified Anti-Human IL-1beta as the detection antibody at a concentration of approximately 0.25-0.50 μg/ml.
Western Blot
To detect Human IL-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-1beta is 2.0-4.0 ng/lane, under reducing conditions and 4.0-8.0 ng/lane, under non-reducing conditions.
Purification:
IL-1 beta-specific antibody was purified from cell culture by Protein G affinity chromatography
Clonality:
Monoclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
IL1B
Additional Names:
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)
Protein Accession Number:
P01584
Protein Gi Number:
P01584.2
Ncbi Gene Id Number:
3553
User Note:
Centrifuge vial prior to opening.
Background:
IL-1 beta is a proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. Both IL-1 alpha and IL-1 beta bind to the same receptor and have similar, if not identical, biological properties. These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine. Recombinant Human IL-1 beta is a 17.3 kDa protein containing 153 amino acid residues.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

38-306

List Size:
0.5 mg

List Price:

$366.00

Datasheet
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