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To a sandwich ELISA (assuming 100ul/well), a concentration of 8.0-16.0 ug/ml of this antibody will detect at least 500 pg/ml of recombinant Rat IL-10 when used with ProSci’s Biotinylated Antigen Affinity Purified Anti-Rat IL-10 (XP-5163Bt) as the detection antibody at a concentration of approximately 0.5-1.0 ug/ml.
To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
To a sandwich ELISA (assuming 100ul/well), a concentration of 8.0-16.0 ug/ml of this antibody will detect at least 500 pg/ml of recombinant Rat IL-10 when used with ProSci’s Biotinylated Antigen Affinity Purified Anti-Rat IL-10 (XP-5163Bt) as the detection antibody at a concentration of approximately 0.5-1.0 ug/ml.

IL-10 Antibody [2F4-11-1]

CATALOG NUMBER: 38-302

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Rat
Immunogen:
E.coli derived Recombinant Rat IL-10
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μL/well), a concentration of 8.0-16.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant Rat IL-10 when used with ProSci’s Polyclonal Biotinylated Antigen Affinity Purified Anti-Rat IL-10 (XP-5163Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Purification:
This antibody was purified from tissue culture by Protein A affinity chromatography.
Clonality:
Monoclonal
Physical State:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
IL10
Additional Names:
Interleukin 10Interleukin-10, Cytokine synthesis inhibitory factor, IL-10, CSIF, TGIF, GVHDS, IL10A
Protein Accession Number:
NP_000563.1
Protein Gi Number:
10835141
Ncbi Gene Id Number:
3586
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-302

List Size:
0.5 mg

List Price:

$285.00

Datasheet
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