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In a sandwich ELISA (assuming 100 μl/well), a concentration of 8.0-10.0 μg/ml of this antibody will detect at least 2000pg/ml of Recombinant Human IL-10 when used with ProSci's Biotinylated Antigen Affinity Purified anti-Human IL-10 as the detection antibody at a concentration of approximately 0.05- 0.10 μg/ml.
To detect Human IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-10 is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.
In a sandwich ELISA (assuming 100 μl/well), a concentration of 8.0-10.0 μg/ml of this antibody will detect at least 2000pg/ml of Recombinant Human IL-10 when used with ProSci's Biotinylated Antigen Affinity Purified anti-Human IL-10 as the detection antibody at a concentration of approximately 0.05- 0.10 μg/ml.

IL-10 Antibody

CATALOG NUMBER: 38-312

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Produced in BALB/c mice immunized with highly pure Recombinant Human IL-10. Anti-Human IL-10-specific antibody was purified from tissue culture supernatant by Protein A affinity chromatography.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Sandwich ELISA: In a sandwich ELISA (assuming 100 μl/well), a concentration of 8.0-10.0 μg/ml of this antibody will detect at least 2000pg/ml of Recombinant Human IL-10 when used with ProSci’s Biotinylated Antigen Affinity Purified anti-Human IL-10 as the detection antibody at a concentration of approximately 0.05- 0.10 μg/ml.
Western Blot
To detect Human IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-10 is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.
Purification:
IL-10-specific antibody was purified from tissue culture supernatant by Protein A affinity chromatography
Clonality:
Monoclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
IL10
Additional Names:
B-TCGF, CSIF, TGIF
Protein Accession Number:
P22301
Protein Gi Number:
P22301.1
Ncbi Gene Id Number:
3586
User Note:
Centrifuge vial prior to opening.
Background:
IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-alpha. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 is active on murine cells, but murine IL-10 is inactive on human cells. Recombinant Human IL-10 is an 18.6 kDa protein of 161 amino acid residues.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

38-312

List Size:
0.5 mg

List Price:

$366.00

Datasheet
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