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Western blot analysis using IL - 10 monoclonal antibody against truncated IL - 10 recombinant protein.
Western blot analysis using IL - 10 monoclonal antibody against truncated IL - 10 recombinant protein.

IL-10 Antibody [3C12C12]

CATALOG NUMBER: 32-227

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified recombinant human IL-10 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:1:500 - 1,000
ELISA:Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein A affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG1.kappa
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
to IL-10 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL10
Additional Names:
Cytokine synthesis inhibitory factor, IL-10, CSIF, TGIF, GVHDS, IL10A
Protein Accession Number:
P22301
Protein Gi Number:
124292
Ncbi Gene Id Number:
3586
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Interleukine 10 (IL-10) is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down- regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Background Reference 1:
Vieira P, et al. PNAS, 1991.88:1172-1176.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-227

List Size:
0.1 mg

List Price:

$345.00

Datasheet
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