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IL-10 Recombinant Protein

CATALOG NUMBER: 40-417

Specifications
source-species:
E. coli
Species:
Murine
source-species:
E. coli
Recombinant Protein Sequence:
MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S
Biological Activity:
Murine IL - 10 is fully biologically active as measured by the dose - dependent co - stimulation (with IL - 4) of the proliferation of murine MC/9 cells. The ED50 was found to be < 2 ng/mL, corresponding to a specific activity of > 5 x 105 units/mg.
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin level is less than 0.1 ng per μg (1EU/μg).
physical-state:
Lyophilized
Storage Conditions:
The lyophilized IL-10 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted IL-10 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing IL-10 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Ncbi Official Symbol:
Il10
Additional Names:
CSIF, Il-10, Interleukin-10, Cytokine synthesis inhibitory factor, IL-10
Protein Accession Number:
NP_034678.1
Protein Gi Number:
6754318
Ncbi Gene Id Number:
16153
Background:
IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNFα. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. Recombinant murine IL-10 is an 18.7 kDa protein of 161 amino acid residues.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

40-417

List Size:
0.002 mg, 0.01 mg

List Price:

Price range: $215.00 through $323.00

Datasheet
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