<b> Immunofluorescence staining of fixed RAW264.7 cells with anti-IL-10R antibody 1B1.3a (</b> Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of 1B1.3a (24-729) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-729- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

<b> Flow cytometry using the Anti-IL-10R antibody 1B1.3a ( </b> RAW264.7 cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of 1B1.3a (24-729- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed RAW264.7 cells with anti-IL-10R antibody 1B1.3a ( Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of 1B1.3a (24-729) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-729- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

IL-10R Antibody [1B1.3a], Rabbit IgG

CATALOG NUMBER: 24-729

Clonality:

Recombinant Monoclonal

Tested Applications:

Blocking, Flow

Species Reactivity:

Mouse

Conjugate:

Unconjugated

Specifications

Species Reactivity:

Mouse

Species:

Rabbit

Original Species Name:

Rat

Chimeric Use Statement:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Immunogen:

This antibody was raised by immunising rats with the purified recombinant extracellular region of mouse Interleukin 10 Receptor.

Conjugate:

Unconjugated

Tested Applications:

Blocking, Flow

Advanced Validation

Properties

Clonality:

Recombinant Monoclonal

Isotype:

IgG kappa

Buffer:

PBS with 0.02% Proclin 300.

Concentration:

batch dependent

Storage Conditions:

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Additional Info

Ncbi Official Symbol:

ADRB2

Additional Names:

CD210, Interleukin-10 receptor subunit, Interleukin-10 receptor subunit alpha, Il10ra Il10r, IL-10 receptor subunit alpha, IL-10R subunit alpha, IL-10RA, CDw210a, Interleukin-10 receptor subunit 1, IL-10R subunit 1, IL-10R1, IBI-3A, 1B1-3A

Ncbi Gene Id Number:

16154

User Note:

For research use only.

Background

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-729

List Size:

0.2 mg

List Price:

$598.00

Datasheet

Shipping Info

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IL-10R Antibody [1B1.3a], Rabbit IgG

Clonality:

Tested Applications:

Species Reactivity:

Conjugate:

Species Reactivity:

Species:

Original Species Name:

Chimeric Use Statement:

Immunogen:

Conjugate:

Tested Applications:

Clonality:

Isotype:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Ncbi Gene Id Number:

User Note:

Disclaimer:

List Size:

Shipping Info

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