Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000pg/ml Recombinant Human IL-12 when used with ProSci's Biotinylated Antigen Affinity Purified Anti-Human IL-12 as the detection antibody at a concentration of at least 0.25-0.5 μg/ml.
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000pg/ml Recombinant Human IL-12 when used with ProSci's Biotinylated Antigen Affinity Purified Anti-Human IL-12 as the detection antibody at a concentration of at least 0.25-0.5 μg/ml.

IL-12 Antibody

CATALOG NUMBER: 38-309

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Produced in BALB/c x ICR F1 mice immunized with highly pure Recombinant Human IL-12. Anti-Human IL- 12-specific antibody was purified from ascites fluid by Protein A affinity chromatography.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000pg/ml Recombinant Human IL-12 when used with ProSci’s Biotinylated Antigen Affinity Purified Anti-Human IL-12 as the detection antibody at a concentration of at least 0.25-0.5 μg/ml.
Western Blot
To detect Human IL-12 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-12 is 0.50-1.0 ng/lane, under non-reducing conditions.
Purification:
IL- 12-specific antibody was purified from ascites fluid by Protein A affinity chromatography
Clonality:
Monoclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
IL12A , IL12B
Additional Names:
Interleukin-12, NKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSFp35: Interleukin-12 subunit alpha, IL-12 subunit p35, IL-12A, Cytotoxic Lymphocyte Maturation Factor 35 kDa subunit (CLMF p35), NK cell Stimulating Factor Chain 1p40: Interleukin-12 subunit beta, IL-12 subunit p40, IL-12B, CytotoxicLymphocyte Maturation Factor 40 kDa subunit (CLMF p40), NK cell Stimulating Factor Chain 2
Protein Accession Number:
p35: P29459 p40: P29460
Protein Gi Number:
P29459.2, P29460.1
Ncbi Gene Id Number:
p35: 3592 p40: 3593
User Note:
Centrifuge vial prior to opening.
Background:
IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-gamma-producing Th1 cells. Recombinant Human IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

38-309

List Size:
0.5 mg

List Price:

$366.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369