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In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Murine IL-12 when used with ProSci's Biotinylated Antigen Affinity Purified anti-Murine IL-12 as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml.
To detect Murine IL-12 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-12 is 1.0-2.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions.
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Murine IL-12 when used with ProSci's Biotinylated Antigen Affinity Purified anti-Murine IL-12 as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml.

IL-12 Antibody

CATALOG NUMBER: 38-311

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rat
Species Reactivity:
Mouse
Immunogen:
Produced in Sprague Dawley rats immunized with highly pure Recombinant Murine IL-12. Anti-Murine IL-12-specific antibody was purified from cell culture by Protein G affinity chromatography.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Murine IL-12 when used with ProSci’s Biotinylated Antigen Affinity Purified anti-Murine IL-12 as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml.
Western Blot
To detect Murine IL-12 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-12 is 1.0-2.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions.
Purification:
IL-12-specific antibody was purified from cell culture by Protein G affinity chromatography
Clonality:
Monoclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Il12a , Il12b
Additional Names:
NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF
Protein Accession Number:
p35: P43431 p40: P43432
Protein Gi Number:
P43431.1, P43432.1
Ncbi Gene Id Number:
p35: 16159 p40: 16160
User Note:
Centrifuge vial prior to opening.
Background:
IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-gamma-producing Th1 cells. Recombinant Murine IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (506 total amino acid residues).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

38-311

List Size:
0.5 mg

List Price:

$366.00

Datasheet
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