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Western blot analysis of IL-12A in HeLa cell lysate with IL-12A antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of IL-12A in human pancreas tissue with IL-12A Antibody at 5 μg/mL.
Immunofluorescence of IL-12A in human pancreas tissue with IL-12A Antibody at 20 μg/mL.
Western blot analysis of IL-12A in HeLa cell lysate with IL-12A antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

IL-12A Antibody

CATALOG NUMBER: 7497

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Llama: (94%), Goat: (89%), Pig: (89%), Sheep: (89%), Horse: (83%), Bovine: (83%)
Immunogen:
IL-12A antibody was raised against an 18 amino acid peptide near the carboxy terminus of human IL-12A.
The immunogen is located within the last 50 amino acids of IL-12A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
IL-12A antibody can be used for detection of IL-12A by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
IL-12A antibody is human specific. IL-12A antibody will not cross-react with IL-12B.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
Predicted: 22 kDa
Observed: 22 kDa
Purification:
IL-12A antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-12A antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-12A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
IL 12A
Additional Names:
Interleukin 12 alpha, p35, natural killer cell stimulatory factor 1, NKSF1, cytotoxic lymphocyte maturation factor 1, CLMF, NF cell stimulatory factor chain 1, NFSK
Protein Accession Number:
NP_000873
Protein Gi Number:
24430219
Ncbi Gene Id Number:
3592
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-12A is subunit of the cytokine IL-12 which acts on T- and natural killer cells, and has a broad array of biological activities. IL-12 is a disulfide-linked heterodimer composed of the 35-kD subunit and a 40-kD subunit that is a member of the cytokine receptor family (1). IL-12 is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells (1,2). The responses of lymphocytes to IL-12 are mediated by the activator of transcription protein STAT4 (2). Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity (3).
Background Reference 1:
Gee K, Guzzo C, Che Mat NF, et al. The IL-12 family of cytokines in infection, inflammation and autoimmune disorders. Inflamm. Allergy Drug Targets 2009; 8:40-52.
Background Reference 2:
Sinigaglia F, D'Ambrosio D, Panina-Bordignon P, et al. Regulation of the IL-12/IL-12R axis: a critical step in T-helper cell differentiation and effector function. Immunol. Rev. 1999; 170:65-72.
Background Reference 3:
Bogdan C, Rollinghoff M, and Diefenbach A. The role of nitric oxide in innate immunity. Immunol. Rev. 2000; 173:17-26.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7497

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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