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To detect hIL-13 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human IL-13 (XP-5167) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-13.
To detect hIL-13 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human IL-13 (XP-5167) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-13.

IL-13 Antibody (biotin)

CATALOG NUMBER: XP-5167Bt

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-13 (human Interleukin-13).
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect hIL-13 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-13 (XP-5167) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-13.
Western Blot:
To detect hIL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-13 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hIL-13 specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
IL-13 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
IL13
Additional Names:
P600, IL-13, NC30, Interleukin-13IL-13
Protein Accession Number:
P35225
Protein Gi Number:
239938644
Ncbi Gene Id Number:
3596
User Note:
Centrifuge vial prior to opening.
Background:
Human IL13 was originally identified by differential screening of an anti CD28 activated human peripheral blood mononuclear cell cDNA library as an induction specific novel cytokine. It was also isolated from cDNA libraries of human T cell clones using the murine IL13 (P600) cDNA as a probe. Human IL13, a pleiotropic cytokine, is produced by activated Th0, Th1 like, Th2 like, and CD8 T cells. The gene for human IL13 maps to chromosome 5 and is closely linked to the genes for IL3, IL4, IL5, and GMCSF.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5167Bt

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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