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To detect hIL-15 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-15 (XP-5169Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-15.
To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.5 mg/ml-1.0 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.5 mg/ml-1.0 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.5 mg/ml-1.0 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To detect hIL-15 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-15 (XP-5169Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-15.

IL-15 Antibody

CATALOG NUMBER: XP-5169

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-15 (human Interleukin-15).
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-15 (2.0 ng/mL), a concentration of 0.12 - 0.15 μg/mL of this antibody is required.
ELISA:
To detect hIL-15 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-15.
To detect hIL-15 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-15 (XP-5169Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-15.
Western Blot:
To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hIL-15 specific antibody was purified by affinity chromatography employing immobilized hIL-15 matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
IL-15 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
IL2RB
Additional Names:
CD122, IL15RB, P70-75Interleukin-15IL-15
Protein Accession Number:
P40933-2
Protein Gi Number:
729830
Ncbi Gene Id Number:
3560
User Note:
Centrifuge vial prior to opening.
Background:
IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5169

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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