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Western blot analysis of IL-15 in rat spleen tissue lysate with IL-15 antibody at (A) 1 and (B) 2 μg/mL
Immunohistochemistry of IL-15 in human spleen tissue with IL-15 antibody at 2.5 μg/ml.
Western blot analysis of IL-15 in rat spleen tissue lysate with IL-15 antibody at (A) 1 and (B) 2 μg/mL
Trial Size

IL-15 Antibody

CATALOG NUMBER: 7231

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Guinea pig: (100%), Sheep: (92%), Rabbit: (92%), Pig: (92%), Bovine: (92%)
Immunogen:
Rabbit polyclonal IL-15 antibody was raised against a 13 amino acid peptide near the center of human IL-15.
The immunogen is located within amino acids 50 - 100 of IL-15.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
IL-15 antibody can be used for detection of IL-15 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of IL-15 are known to exist.
Positive Control 1:
Cat. No. 1466 - Rat Spleen Tissue Lysate
Predicted Molecular Weight:
18 kDa
Purification:
IL-15 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-15 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
IL15
Additional Names:
IL-15 Antibody: IL-15, Interleukin-15, IL-15
Protein Accession Number:
NP_000576
Protein Gi Number:
10835153
Ncbi Gene Id Number:
3600
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-15 Antibody: Interleukin 15 (IL-15) is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis.
Background Reference 1:
Bodnar A, Nizsaloczki E, Mocsar G, et al. A biophysical approach to IL-2 and IL-15 receptor function: localization, conformation and interactions. Immunol. Lett. 2008; 116:117-25.
Background Reference 2:
Ku CC, Murakami M, Sakamoto A, et al. Control of homeostasis of CD8+ memory T cells by opposing cytokines. Science 2000; 288:675-8.
Background Reference 3:
Waldmann T, Tagaya Y, and Bamford R. Interleukin-2, interleukin-15, and their receptors. Int. Rev. Immunol. 1998; 16:205-26.
Background Reference 4:
Masuda A, Matsuguchi T, Yamaki K, et al. Interleukin-15 prevents mouse mast cell apoptosis through STAT6-mediate Bcl-xL expression. J. Biol. Chem. 2001; 276:26107-13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7231

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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