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IL-15 Antibody [PEPY024_hIL-15]

CATALOG NUMBER: XP-5709-M

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
The monoclonal antibody was produced in BALB/c x C57BL/6 F1 mice using recombinant hIL-15 as the immunizing antigen.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 250 pg/mL of recombinant human IL-15 when used with our biotinylated antigen affinity purified anti-human IL-15 (XP-5169Bt) as the detection antibody at a concentration of approximately 2.0-4.0 μg/mL.
Western Blot:
To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/mL (non-reducing) or at a concentration of 2.0-4.0 μg/mL (reducing). Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Purification:
This IgG1/k antibody was purified from ascites fluid on a Protein A affinity column.
Clonality:
Monoclonal
Physical State:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
IL-15 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
Ncbi Official Symbol:
IL15
Additional Names:
Interleukin 15Interleukin-15IL-15, IL-15
Protein Accession Number:
P40933
Protein Gi Number:
729830
Ncbi Gene Id Number:
3600
User Note:
Centrifuge vial prior to opening.
Background:
IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97% and 73% amino acid sequence identity with simian and mouse IL15, respectively. IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5709-M

List Size:
0.5 mg

List Price:

$325.00

Out of stock

Datasheet
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