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Western blot analysis of IL-15RA in rat small intestine tissue lysate with IL-15RA antibody at 1 μg/mL.
Western blot analysis of IL-15RA in rat small intestine tissue lysate with IL-15RA antibody at 1 μg/mL.
Trial Size

IL-15RA Antibody

CATALOG NUMBER: 7233

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rabbit polyclonal IL-15RA antibody was raised against a 16 amino acid peptide near the center of human IL-15RA.
The immunogen is located within amino acids 40 - 90 of IL-15RA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
IL-15RA antibody can be used for detection of IL-15RA by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in rat samples. All other applications and species not yet tested.
Specificity:
Multiple isoforms of IL-15RA are known to exist.
Positive Control 1:
Cat. No. 1468 - Rat Small Intestine Tissue Lysate
Predicted Molecular Weight:
Predicted: 26, 30, 38 kDa
Observed: 30 kDa
Purification:
IL-15RA Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-15RA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-15RA antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
IL15RA
Additional Names:
IL-15RA Antibody: CD215, Interleukin-15 receptor subunit alpha, IL-15 receptor subunit alpha
Protein Accession Number:
NP_001243694
Protein Gi Number:
377520151
Ncbi Gene Id Number:
3601
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-15RA Antibody: Interleukin 15 (IL-15RA) is a cytokine receptor that specifically binds IL-15 with high affinity. IL-15 regulates T and natural killer cell activation and proliferation. The IL-15 and IL-2 receptors share two subunits, IL-2R beta and IL-2R gamma, and IL-15RA is structurally related to IL-2RA, an additional IL-2-specific alpha subunit necessary for high affinity IL-2 binding. Unlike IL-2RA, IL-15RA is capable of binding IL-15 with high affinity independent of the other subunits. IL-15RA is thought to enhance cell proliferation and expression of apoptosis inhibitor Bcl-xL and Bcl-2.
Background Reference 1:
Giri JG, Kumaki S, Ahdieh M, et al. Identification and cloning of a novel IL-15 binding protein that is structurally related to the alpha chain of the IL-2 receptor. EMBO J. 1995; 14:3654-63.
Background Reference 2:
Bodnar A, Nizsaloczki E, Mocsar G, et al. A biophysical approach to IL-2 and IL-15 receptor function: localization, conformation and interactions. Immunol. Lett. 2008; 116:117-25.
Background Reference 3:
Lorenz HM, Hieronymus T, Grunke M, et al. Differential role for IL-2 and IL-15 in the inhibition of apoptosis in short-term activated human lymphocytes. Scand. J. Immunol. 1997; 45:660-9.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7233

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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