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Western blot analysis of IL-16 in rat brain tissue lysate with IL-16 antibody at (A) 1 μg/ml and (B) 2 μg/mL.
Immunohistochemistry of IL-16 in mouse brain tissue with IL-16 antibody at 2.5 μg/mL.
Immunofluorescence of IL-16 in mouse brain tissue with IL-16 antibody at 20 μg/mL.
Western blot analysis of IL-16 in rat brain tissue lysate with IL-16 antibody at (A) 1 μg/ml and (B) 2 μg/mL.
Trial Size

IL-16 Antibody

CATALOG NUMBER: 4985

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
IL-16 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human IL-16.
The immunogen is located within the first 50 amino acids of IL-16.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
IL-16 antibody can be used for detection of IL-16 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
This antibody will only detect the NIL-16 isoform.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
IL-16 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-16 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-16 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL16
Additional Names:
IL-16 Antibody: LCF, NIL16, PRIL16, prIL-16, Pro-interleukin-16, Lymphocyte chemoattractant factor, IL-16
Protein Accession Number:
AAQ86961
Protein Gi Number:
36953836
Ncbi Gene Id Number:
3603
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-16 Antibody: IL-16 was initially identified as a chemotactic cytokine, but is now known to possess a wide range of activities. Later studies have more fully characterized IL-16 as an immunomodulatory cytokine that contributes to the regulatory process of CD4+ T cell recruitment and activation at sites of inflammation in association with asthma and several autoimmune diseases. The precursor of IL-16 (pro-IL-16) is thought to be cleaved towards the C-terminal region by Caspase-3, releasing a 20 kDa active form that binds to and signals through CD4. Besides acting as a chemotactic cytokine, IL-16 is thought to also be involved in the regulation of T cell proliferation and multiple infectious, immune-mediated, and autoimmune inflammatory disorders including irritable bowel syndrome, systemic lupus erythematosus, and neurodegenerative disorders. At least two isoforms of IL-16 are known to exist; the longer isoform (also known as NIL-16) is detected only in neurons of the cerebellum and hippocampus.
Background Reference 1:
Cruikshank WW, Center DM, Nisar N, et al. Molecular and functional analysis of a lymphocyte chemoattractant factor: association of biologic function with CD expression. Proc. Natl. Acad. Sci. USA1994; 91:5109-13.
Background Reference 2:
Interleukin-16. Cruikshank WW, Kornfeld H, and Center DM. J. Leukoc. Biol.2000; 67:757-66.
Background Reference 3:
Zhang Y, Center DM, Wu DM, et al. Processing and activation of pro-interleukin-16 by caspase-3. J. Biol. Chem.1998; 273:1144-9.
Background Reference 4:
Maciaszek JW, Parada NA, Cruikshank WW, et al. IL-16 represses HIV-1 promoter activity. J. Immunol.1997; 158:5-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4985

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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