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<strong>Figure 1 Western Blot Validation in Human THP-1 Cell Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IL-17 4877 (A: 1 μg/mL and B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in A-20 Cell Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IL-17 4877 (A: 2 μg/mL and B: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Recombinant Protein Test </strong><br>Loading: 30 ng of human IL-17 recombinant protein per lane.Antibodies: IL-17 4877, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.125 μg/mLLane 2: 0.25 μg/mLLane 3: 0.5 μg/mL
Figure 1 Western Blot Validation in Human THP-1 Cell Lysate
Loading: 15 μg of lysates per lane.Antibodies: IL-17 4877 (A: 1 μg/mL and B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

IL-17 Antibody

CATALOG NUMBER: 4877

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
IL-17 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-17A.
The immunogen is located within amino acids 40 - 90 of IL-17.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 0.125-4 μg/mL.
Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1208 - THP-1 Cell Lysate
Positive Control 2:
Cat. No. 1288 - A20 Cell Lysate
Predicted Molecular Weight:
Predicted: 17.5kD
Observed: 20 kD (Post-modification: 1 N-linked glycosylation)
Validation:

Recombinant Protein Test (Figure 3): Anit-IL-17 antibodies (4877) detected human IL-17 recombinant protein at different concentrations.

Isoforms:
Human IL-17 has only one isoform (155aa, 17.5kD). Mouse IL-17 has one isoform (158aa, 17.5kD) and rat IL-17 also has one isoform (150aa, 16.9kD). 4877 can detect only human isoform, but cannot detect mouse and rat.
Purification:
IL-17 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-17 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-17 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL17A
Additional Names:
IL-17 Antibody: IL17, CTLA8, IL-17, IL-17A, IL17, Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8
Protein Accession Number:
NP_002181
Protein Gi Number:
4504651
Ncbi Gene Id Number:
3605
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-17 Antibody: Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-κB and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.
Background Reference 1:
Miossec. Curr. Opin. Rheumatol.2000; 12:181-5.
Background Reference 2:
Paunovic et al. Rheumatol.2008; 47:771-6.
Background Reference 3:
Steinman. Nat. Med.2007; 13:139-145.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4877

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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