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<strong>Figure 1 Western Blot Validation with Recombinant Protein</strong><br>Loading: 30 ng of human IL-17 recombinant protein per lane.Antibodies: IL-17 4887, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.125 μg/mLLane 2: 0.25 μg/mLLane 3: 0.5 μg/mL
<strong>Figure 2 Western Blot Validation with Human Spleen </strong><br>Loading: 10 μg of lysates per lane.Antibodies: IL-17 4887, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 1 minLane 1: 1 μg/mL Lane 2: 2 μg/mL
<strong>Figure 3 Immunohistochemistry Validation of IL-17 in Human Lymph Node </strong><br> Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-IL-17 antibody (4887) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 4 Immunofluorescence Validation of IL-17 in Mouse Heart Tissue</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart issue labeling IL-17 with 4887 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5 Immunofluorescence Validation of IL-17 in Mouse A-20 Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse A-20 Cells labeling IL-17 with 4887 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 6 Immunofluorescence Validation of IL-17 in Mouse Thymus Tissue</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus tissue labeling IL-17 with 4887 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
<strong>Figure 7 Immunohistochemistry Validation of IL-17 in Mouse Colon Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IL-17 antibody (4887) at 2 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4° C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 8 Immunohistochemistry Validation of IL-17 in Human Tonsil Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-17 antibody (4887) at 5 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4° C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 Western Blot Validation with Recombinant Protein
Loading: 30 ng of human IL-17 recombinant protein per lane.Antibodies: IL-17 4887, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.125 μg/mLLane 2: 0.25 μg/mLLane 3: 0.5 μg/mL
Trial Size

IL-17 Antibody

CATALOG NUMBER: 4887

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (84%), Rat: (81%), Pig: (74%)
Immunogen:
Anti-IL-17 antibody (4887) was raised against a peptide corresponding to 16 amino acids near the center of mature human IL-17.
The immunogen is located within amino acids 50-100 of IL-17.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WB: 0.125-2 μg/mL; IF: 5-20 μg/mL; IHC: 1-5 μg/mL .
Antibody validated: Western Blot in human samples; Immunofluorescence in mouse samples; Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 17-208 - A20 Cell Slide
Predicted Molecular Weight:
Predicted: 17.5kD
Observed: 18 kDa
Validation:

Recombinant Protein Test (Figure 1): Anit-IL-17 antibodies (4887) detected human IL-17 recombinant protein at different concentrations

Isoforms:
Human IL-17 has only one isoform (155aa, 17.5kD). Mouse IL-17 has one isoform (158aa, 17.5kD) and Rat IL-17 also has one isoform (150aa, 16.9kD). 4887 can detect human, mouse and rat IL-17.
Purification:
IL-17 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-17 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-17 antibody can be stored at 4°C for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL17A
Additional Names:
IL-17 Antibody: IL17, CTLA8, IL-17, IL-17A, IL17, Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8
Protein Accession Number:
NP_002181
Protein Gi Number:
4504651
Ncbi Gene Id Number:
3605
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-17 Antibody: Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-κB and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.
Background Reference 1:
Miossec. Curr. Opin. Rheumatol.2000; 12:181-5.
Background Reference 2:
Paunovic et al. Rheumatol.2008; 47:771-6.
Background Reference 3:
Steinman. Nat. Med.2007; 13:139-145.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4887

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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