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Western Blot Validation of IL-17 in Human Tonsil Loading: 10 μg of lysates per lane. Antibodies: IL-17, PM-10543, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution
Western Blot Validation of IL-17 in Mouse Cells and Tissues Loading: 10 μg of lysates per lane. Antibodies: IL-17, PM-10543, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution
Western Blot Validation of IL-17 in Human Tonsil Loading: 10 μg of lysates per lane. Antibodies: IL-17, PM-10543, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution
IAV
Trial Size

IL-17 Antibody [8F4]

CATALOG NUMBER: PM-10544

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC-P, WB, IHC-P/IF
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (84%), Rat: (81%), Pig: (74%)
Immunogen:
Anti-IL-17 antibody was raised against a peptide corresponding to 16 amino acids near the center of mature human IL-17. The immunogen is located within amino acids 50-100 of IL-17.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB, IHC-P/IF
Application Note:
WB: 1-2 μg/mL; IF: 5-20 μg/mL; IHC: 1-5 μg/mL. Antibody validated: Western Blot in human and mouse samples; Immunofluorescence in mouse samples; Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1315 - Human Tonsil Tissue
Predicted Molecular Weight:
Predicted: 17.5 kDa. Observed: 18 kDa
Purification:
IL-17 Antibody is supplied as protein A purified IgG2b
Clonality:
Monoclonal
Isotype:
IgG2b
physical-state:
Liquid
Buffer:
IL-17 Antibody is supplied in PBS containing 0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-17 antibody can be stored at 4°C for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL17A
Additional Names:
IL-17 Antibody: IL17, CTLA8, IL-17, IL-17A, IL17, Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8
Protein Accession Number:
NP_002181
Protein Gi Number:
4504651
Ncbi Gene Id Number:
3605
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-17 Antibody: Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-κB and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.
Background Reference 1:
Miossec. Curr. Opin. Rheumatol.2000; 12:181-5.
Background Reference 2:
Paunovic et al. Rheumatol.2008; 47:771-6.
Background Reference 3:
Steinman. Nat. Med.2007; 13:139-145.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

CATALOG NUMBER:

PM-10544

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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