IL-17F Antibody

Application Note:

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17F (50.0 ng/mL), a concentration of 0.9-1.2 μg/mL of this antibody is required.
ELISA:
Indirect:
To detect hIL-17F by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17F.
Sandwich
To detect hIL-17F by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human IL-17F as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17F.
Western Blot:
To detect hIL-17F by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17F is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.