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<strong> Figure 1 WB Validation in K562 Cells </strong><br>Loading: 10 μg of lysate Antibodies: IL-17RA, 7419, 4 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 WB Validation in Mouse Tissues </strong><br>Loading: 15 μg of lysate Antibodies: IL-17RA, 7419, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Immunofluorescence Validation of IL-17RA in Human Spleen</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling IL-17RA with 7419 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
<strong>Figure 4 Immunofluorescence Validation of IL-17RA in Mouse Thymus</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus tissue labeling IL-17RA with 7419 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Flow Cytometry Validation of IL-17RA in 293 Cells 1X10^6/mL 293 cells were intracellularly stained with 2 μg/mL of IL-17RA antibody (red) or Rabbit IgG isotype control (black) at RT for 30 min, followed by PSFlo 488 conjugated goat anti-rabbit IgG secondary antibody staining (1:1000) at RT for 30 min. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100 in PBS.
Figure 1 WB Validation in K562 Cells
Loading: 10 μg of lysate Antibodies: IL-17RA, 7419, 4 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

IL-17RA Antibody

CATALOG NUMBER: 7419

Clonality:
Polyclonal
Tested Applications:
ELISA, FC, WB, IHC-P/IF
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Anti-IL-17RA antibody (7419) was raised against a peptide corresponding to 18 amino acid synthetic peptide near the amino terminus of human IL-17RA.

The immunogen is located within amino acids 150-200 of IL-17RA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, WB, IHC-P/IF
Application Note:
WB: 1-4 μg/mL; IHC-P/IF: 10-20 μg/mL; FC: 2 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunofluorescence in human and mouse samples; Flow Cytometry in human samples. All other applications and species not yet tested.
Specificity:
IL-17RA antibody is human, mouse and rat reactive. Multiple isoforms of IL-17RA are known to exist. This antibody is predicted to not cross-react with other IL-17 receptor subunits.
Positive Control 1:
Cat. No. 1409 - Mouse Thymus Tissue Lysate
Predicted Molecular Weight:
Predicted: 96 kD

Observed: 100 kD
Purification:
IL-17RA Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-17RA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-17RA antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL17RA
Additional Names:
IL-17RA Antibody: CD217, IL17R, CANDF5, CDw217, IL-17RA, hIL-17R, Interleukin-17 receptor A, IL-17 receptor A
Protein Accession Number:
NP_055154
Protein Gi Number:
23238208
Ncbi Gene Id Number:
23765
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-17RA Antibody: Interleukin 17A (IL-17A) is a proinflammatory cytokine secreted by activated T-lymphocytes (1). It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. IL-17RA is a ubiquitous type I membrane glycoprotein that binds with low affinity to IL-17A and IL-17F (1,2). IL-17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis (3,4). Defects in IL-17RA are the cause of familial candidiasis type 5 (CANDF5) which is a rare disorder with altered immune responses and impaired clearance of fungal infections (5).
Background Reference 1:
Yao Z, Painter SL, Fanslow WC, et al. Human IL-17: a novel cytokine derived from T cells. J. Immunol. 1995; 155: 5483-6.
Background Reference 2:
Yao Z, Fanslow WC, Seldin MF, et al. Herpesvirus saimiri encodes a new cytokine, IL-17, which binds to a novel cytokine receptor. Immunity 1995; 3:811-21.
Background Reference 3:
Rickel EA, Siegel LA, Yoon BR, et al. Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities. J. Immunol. 2008;181:4299-310.
Background Reference 4:
Wang M, Wang L, Ren T, et al. IL-17A/IL-17RA interaction promoted metastasis of osteosarcoma cells. Cancer Biol. Ther. 2012;14(2).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7419

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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