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<b> Western Blot using anti-IL-18 antibody SAIC-24B-4 (</b> Untreated human peripheral blood monocyte (PBM; A) and PMA/lonomycin-treated PBM lysates (35ug protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots probed with the r-chimeric version of SAIC-24B-4 (24-320) at 0.3 ug/ml before detection using an anti-rondary antibody. Protein was detected by chemiluminescence. The expected running size for IL-18 is 22.3kDa. 24-320 successfully detected IL-18 in untreated human peripheral blood monocyte samples.
<b> Flow-cytometry using the anti-IL-18 antibody SAIC-24B-4 (</b> Human peripheral blood monocytes (PBMC's) were fixed using 2% PFA- permeusing 0.5% Triton and stained with unimmunized r antibody (MOPC-21; isotype control- black line) or the r-chimeric version of SAIC-24B-4 (24-320- blue line) at a dilution of 1:100 for 1h at RT. An additional population of PBMC's stimulated with PMA and Ionomycin in the presence of Monensin for 4 hours were stained in a similar manner (purple line). After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Western Blot using anti-IL-18 antibody SAIC-24B-4 ( Untreated human peripheral blood monocyte (PBM; A) and PMA/lonomycin-treated PBM lysates (35ug protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots probed with the r-chimeric version of SAIC-24B-4 (24-320) at 0.3 ug/ml before detection using an anti-rondary antibody. Protein was detected by chemiluminescence. The expected running size for IL-18 is 22.3kDa. 24-320 successfully detected IL-18 in untreated human peripheral blood monocyte samples.

IL-18 Antibody [SAIC-24B-4], Rabbit IgG

CATALOG NUMBER: 24-320

Clonality:
Recombinant Monoclonal
Tested Applications:
immunoMRM
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human
Species:
Rabbit
Original Species Name:
Rabbit
Immunogen:
The KLH conjugated peptide "ISTLSCENK" derived from IL-18, an augmentor of natural killer cell activity in the spleen and stimulator of interferon gamma production in Th1 cells.
Conjugate:
Unconjugated
Tested Applications:
immunoMRM
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
IL18
Additional Names:
Interleukin-18, IGIF, IL1F4
Ncbi Gene Id Number:
3606
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-320

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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