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To detect Rat IL-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat IL-1-alpha (XP-5176Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1-alpha.
To detect Rat IL-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat IL-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained colchicine injected rat brain (including the ventricles and the CA3 region of the hippocampus) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.
This antibody stained colchicine injected rat brain (including the ventricles and the CA3 region of the hippocampus) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.
To detect Rat IL-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat IL-1-alpha (XP-5176Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1-alpha.

IL-1a Antibody

CATALOG NUMBER: XP-5176

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-1alpha (Rat IL-1alpha).
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-1α (50pg/mL), a concentration of 0.011-0.017 μg/mL of this antibody is required.
ELISA:
To detect Rat IL-1α by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat IL-1α.
Sandwich:
To detect Rat IL-1α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IL-1α (XP-5176Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1α.
Western Blot:
To detect Rat IL-1α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-1α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-Rat IL-1alpha specific antibody was purified by affinity chromatography employing immobilized Rat IL-1alpha matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
IL-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Il1a
Additional Names:
IL-1 alphaInterleukin-1 alphaIL-1 alpha
Protein Accession Number:
P16598
Protein Gi Number:
124301
Ncbi Gene Id Number:
24493
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5176

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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