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<strong>Figure 1 Western Blot Validation with Recombinant Protein</strong><br>Loading: 30 ng of human IL-1F10 recombinant protein per lane.Antibodies: IL-1F10 7503 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Liver Tissue Lysate in the (A) absence or (B) presence of blocking peptide</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IL-1F10, 7503 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Immunohistochemistry Validation of IL-1F10 in Human Spleen Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-1F10 antibody (7503) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 Western Blot Validation with Recombinant Protein
Loading: 30 ng of human IL-1F10 recombinant protein per lane.Antibodies: IL-1F10 7503 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

IL-1F10 Antibody

CATALOG NUMBER: 7503

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse (100%) and Rat (88%).
Immunogen:
Anti-IL-1F10 antibody (7503) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human IL-1F10.
The immunogen is located within the last 50 amino acids of IL-1F10.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
WB: 1 μg/mL; IHC: 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
IL-1F10 antibody is human, mouse and rat reactive. Two isoforms of IL-1F10 exist.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 17kD
Observed: 17 kD
Validation:

Recombinant Protein Test (Figure 1): Anti- IL-1F10  antibodies (7503) detected human IL-1F10  recombinant protein at different concentrations.

Isoforms:
Human IL-1F10 has 2 isoforms, including isoform 1 (152aa, 17kD) and isoform 2 (152aa, 17kD). Mouse IL-1F10 has one isoform (152aa, 17kD) and Rat IL-1F10 also has one isoform (152aa, 17kD). 7503 can detect human, mouse and rat.
Purification:
IL-1F10 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-1F10 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-1F10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
IL1F10
Additional Names:
IL-1F10 Antibody: IL-38, FKSG75, IL1HY2, IL-1HY2, IL1-theta, FIL1-theta, FIL1T, IL38, UNQ6119/PRO20041, Interleukin-1 family member 10, Family of interleukin 1-theta, IL-1F10
Protein Accession Number:
NP_775184
Protein Gi Number:
27894307
Ncbi Gene Id Number:
84639
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The cytokine IL-1 is responsible for initiating a variety of activities through the activation of transcription factors, NF-kappa B and AP-1, thereby promoting host response to injury or infection (1). IL-1F10 (Interleukin-1 family member 10), also known as FIL1T, IL1HY2 or FKSG75, is highly expressed in the skin, spleen and proliferating B-cells of the tonsil (2). IL-1F10 binds soluble IL-1 receptor type 1, and is one of nine Interleukin 1 families clustered on chromosome 2, where it is thought to participate in the regulation of adapted and innate immune responses (3). IL-1F10 and IL-1RN polymorphisms may play an important role in the susceptibility to developing rheumatoid arthritis (4).
Background Reference 1:
Towne et al. J. Biol. Chem. 2004; 279:13677-88.
Background Reference 2:
Lin et al. J. Biol. Chem. 2001; 276: 20597-602.
Background Reference 3:
Bensen et al. J. Interferon Cytokine Res. 2001; 21:899-904.
Background Reference 4:
Cruz-Robles et al. Immunol. Invest. 2009; 38:231-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7503

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

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