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Western blot analysis of IL-1RAcP expression in K562 cell lysate with IL-1RAcP antibody at 1 μg/ml.
Immunocytochemistry of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 5 μg/mL.
Western blot analysis of IL-1RAcP expression in K562 cell lysate with IL-1RAcP antibody at 1 μg/ml.
Trial Size

IL-1RAcP Antibody

CATALOG NUMBER: 2131

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (91%), Mouse: (86%)
Immunogen:
IL-1RAcP antibody was raised against a peptide corresponding to 22 amino acids near the carboxy terminus of human IL-1RAcP.
The immunogen is located within the last 50 amino acids of IL-1RAcP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
IL-1RAcP antibody can be used for detection of IL-1RAcP by Western blot 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity:
It has no cross activity to other member in the IL-1 receptor family.
Positive Control 1:
Cat. No. 1204 – K562 Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 63 kDa
Observed: 63 kDa
Purification:
IL-1RAcP Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-1RAcP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-1RAcP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL1RAP
Additional Names:
IL-1RAcP Antibody: IL1R3, C3orf13, IL-1RAcP, IL1R3, Interleukin-1 receptor accessory protein, Interleukin-1 receptor 3, IL-1 receptor accessory protein
Protein Accession Number:
NP_002173
Protein Gi Number:
2599126
Ncbi Gene Id Number:
3556
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-1RAcP Antibody: The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I(IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-κB inducing kinae (NIK) and of NF-κB, recruites to the IL-1R complex through IL-1 RAcP. IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.
Background Reference 1:
Greenfeder SA, Nunes P, Kwee L, et al. Molecular cloning and characterization of a second subunit of the interleukin 1 receptor complex. J. Biol. Chem. 1995;270:13757-65.
Background Reference 2:
Huang J, Gao X, Li S, et al. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin 1 receptor accessory protein. Proc. Natl. Acad. Sci. USA 1997; 94:12829-32.
Background Reference 3:
Wesche H, Korherr C, Kracht M, et al. The interleukin-1 receptor accessory protein (IL-1RAcP) is essential for IL-1-induced activation of interleukin-1 receptor-associated kinase (IRAK) and stress-activated protein kinases (SAP kinases). J. Biol. Chem. 1997;272:7727-31.
Background Reference 4:
Hofmeister R, Wiegmann K, Korherr C, et al. Activation of acid sphingomyelinase by interleukin-1 (IL-1) requires the IL-1 receptor accessory protein. J. Biol. Chem. 1997; 272:27730-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2131

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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