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To detect Rat IL-2 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat IL-2 (38-205) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-2.
To detect Rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat IL-2 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat IL-2 (38-205) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-2.

IL-2 Antibody

CATALOG NUMBER: 38-204

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Rabbit
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Rat
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat IL-2. Rat IL-2 specific antibody was purified by affinity chromatography employing immobilized Rat IL-2 matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-2 (1.0 ng/mL), a concentration of < 0.1 μg/mL of this antibody is required.
ELISA:
Indirect:
To detect Rat IL-2 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-2.
Sandwich
To detect Rat IL-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Rat IL-2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-2.
Western Blot:
To detect Rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
IL-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Il2
Additional Names:
Il-2, Interleukin-2, T-cell growth factor, IL-2
Protein Accession Number:
P17108
Protein Gi Number:
124328
Ncbi Gene Id Number:
116562
User Note:
Centrifuge vial prior to opening.
Background:
Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-204

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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