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In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-2 when used with ProSci's Biotinylated Antigen Affinity Purified Anti-Human IL-2 as the detection antibody at a concentration of approximately 1.0- 2.0 μg/ml.
To detect Human IL-2 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-2 when used with ProSci's Biotinylated Antigen Affinity Purified Anti-Human IL-2 as the detection antibody at a concentration of approximately 1.0- 2.0 μg/ml.

IL-2 Antibody

CATALOG NUMBER: 38-307

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Produced in mice immunized with highly pure Recombinant Human IL-2. Anti-Human IL-2-specific antibody was purified from cell culture by Protein A affinity chromatography.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-2 when used with ProSci’s Biotinylated Antigen Affinity Purified Anti-Human IL-2 as the detection antibody at a concentration of approximately 1.0- 2.0 μg/ml.
Western Blot
To detect Human IL-2 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Purification:
IL-2-specific antibody was purified from cell culture by Protein A affinity chromatography
Clonality:
Monoclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
IL2
Additional Names:
Interleukin-2, T-cell growth factor (TCGF), Aldesleukin
Protein Accession Number:
P60568
Protein Gi Number:
P60568.1
Ncbi Gene Id Number:
3558
User Note:
Centrifuge vial prior to opening.
Background:
IL-2 is a powerful immunoregulatory lymphokine produced by T cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant Human IL-2 is a 15.5 kDa protein, containing 134 amino acid residues including one intrachain disulfide bond.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

38-307

List Size:
0.5 mg

List Price:

$366.00

Datasheet
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