Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

<strong>Figure 1 Western Blot Validation in Human, Mouse and Rat Tissues</strong><br>Loading: 15 µg of lysates per lane.Antibodies: IL-21 2465, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP
<strong>Figure 2 Western Blot Validation with Recombinant Protein</strong><br>Loading: 30 ng of human IL-21 recombinant protein per lane.Antibodies: IL-21 2465 (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Immunohistochemistry Validation of IL-21 in Human Tonsil</strong><br> Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-21 antibody (2465) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 4 Immunohistochemistry Validation of IL-21 in Mouse Spleen</strong><br> Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-IL-21 antibody (2465) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 5 Immunohistochemistry Validation of IL-21 in Rat Spleen</strong><br> Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-IL-21 antibody (2465) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Induced Expression Validation of IL-21 expresssion in patients with Crohn's Disease (Monteleone et al, 2007) </strong><br>Enhanced IL-21 was observed in involved but not uninvolvedCD, and was not associated with any CD phenotype, such as fibrostenosing disease. IL-21 expression was detected by anti-IL-21 antibodies (2465).
<strong>Figure 7 Regulation of IL-21 expresssion in duodenal biopsies of two patients with active Celiac disease (ACD) (Sarra et al., 2013) </strong><br>(b) shows IL-21 expression levels treated with dimethyl sulfoxide (DMSO) or wortmannin (WRT). (c) shows IL-21 expression levels with the inhibition of IL-15 antibody (aIL-15). IgG isotype was used as a control.Both WB show IL-21 expression decreased with the treatment with WRT or the inhibition of IL-15 antibody.
<strong>Figure 8 Induced Expression Validation of IL-21 expresssion in patients with Helicobacter Pylori (Hp) (Caruso et al., 2007) </strong><br>(A) shows IL-21 expression levels from biopsies of patients with Hp infection and without Hp infection (n=3).(C) shows IL-21 expression levels in CD3+LPMC cells from Hp-positive and Hp-negative patients (n=3).Both WB show IL-21 expression increased in patients with Hp infection.
Figure 1 Western Blot Validation in Human, Mouse and Rat Tissues
Loading: 15 µg of lysates per lane.Antibodies: IL-21 2465, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP
Green Product
IAV
Most Cited
Trial Size

IL-21 Antibody

CATALOG NUMBER: 2465

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (87%)
Immunogen:
Anti-IL-21 antibody (2465) was raised against a peptide corresponding to 15 amino acids near the center of human IL-21 precursor.
The immunogen is located within the last 50 amino acids of IL-21.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
WB: 0.5-2 μg/mL; IHC-P: 0.5-2 μg/mL.

Antibody validated: Western Blot and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of IL-21 are known to exist; this antibody will detect both isoforms. IL-21 antibody is predicted to not cross-react with other members of the interleukin protein family.
Positive Control 1:
Cat. No. 1315 – Human Tonsil Lysate
Positive Control 2:
Cat. No. 1406 – Mouse Spleen Lysate
Positive Control 3:
Cat. No. 1466 – Rat Spleen Lysate
Predicted Molecular Weight:
Predicted: 18kD
Observed: 17 kD
Validation:

Independent Antibody Validation in Cell Lines (Figure 2) shows similar IL-21 expression profile in human and mouse cell lines detected by two independent anti-IL-21 antibodies that recognize different epitopes, 2465 against central domain and 2463 against C-terminus domain.  IL-21 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

Recombinant Protein Test (Figure 2): Anit-IL-21 antibodies (2465) detected human IL-21 recombinant protein.

Regulated expression validation (Figure 6, 7, 8): IL-21 expression detected by anit-IL-21 antibodies (2465) was up down-regulated by WRT treatment (figure 7) or was up-regulated in Hp positive patients (figure 8) and in patients with Crohn’s disease (figure 6).

Isoforms:
Human IL-21 has 2 isoforms, including isoform 1 (162aa, 18.7kD) and isoform 2 (153aa, 17.6kD). Mouse IL-21 has one isoform (146aa, 16.8kD) and Rat IL-21 also has one isoform (146aa, 17.1kD). 2465 can detect both human isoforms and also detect mouse and rat.
Purification:
IL-21 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
IL-21 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
IL-21 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL21
Additional Names:
IL-21 Antibody: Za11, IL-21, Interleukin-21, Za11
Protein Accession Number:
NP_068575
Protein Gi Number:
11141875
Ncbi Gene Id Number:
59067
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-21 Antibody: A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Background Reference 1:
Parrish-Novak et al. Nature 2000; 408:57-63.
Background Reference 2:
Ozaki et al. Proc. Natl. Acad. Sci. USA. 2000; 97:11439-44.
Background Reference 3:
Asao et al. J. Immunol. 2001; 167:1-5.
Citation 1:
Monteleone et al. Interleukin-21 enhances T-helper cell type I signaling and interferon-γ production in Crohn's disease. Gastroenterology. 2005;128(3):687-94. PMID: 15765404
Citation 2:
Sarra et al. IL-15 positively regulates IL-21 production in celiac disease mucosa. Mucosal Immunol. 2013;6(2):244-55. PMID: 22785229
Citation 3:
Caruso et al. IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis. J Immunol. 2007;178(9):5957-65. PMID: 17442980
Citation 4:
Daniele Fina. Interleukin-21 controls inflammatory pathways in IBD. University of Rome, Italy. PhD thesis. 2009.PMID:
Citation 5:
Roberta Caruso. Expression and functional role of Th-17 cells in helicobacter pylori-related gastritis. University of Rome, Italy. PhD thesis. 2008.PMID:
Citation 6:
Giovanni Monteleone. Antigenicepitopes of Interleukin-21, related antibodies and their use in medical field. U.S patent application publication. 2009.PMID:

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2465

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369