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To detect mIL-21 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-21 (38-213) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21.
To detect mIL-21 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-21 (38-213) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21.

IL-21 Antibody

CATALOG NUMBER: 38-212

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-21 (Murine Interleukin-21). Murine IL-21 specific antibody was purified by affinity chromatography employing immobilized mIL-21 matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Indirect:
To detect mIL-21 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21.
Sandwich
To detect mIL-21 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-21 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21.
Western Blot:
To detect mIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
IL-21 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Il21
Additional Names:
Interleukin-21IL-21
Protein Accession Number:
Q9ES17
Protein Gi Number:
55976590
Ncbi Gene Id Number:
60505
User Note:
Centrifuge vial prior to opening.
Background:
A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-212

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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