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Western blot analysis of IL-21 receptor expression in human HepG2 cell lysate with IL-21 receptor antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of IL-21 receptor in rat lung with IL-21 receptor antibody at 10 μg/mL.
Immunofluorescence of IL-21 Receptor in Rat Lung cells with IL-21 Receptor antibody at 20 μg/mL.
Immunofluorescence of IL21R in A431 cells with IL 21 Receptor antibody at 20 μg/mL.<br><br>Green: IL-21 Receptor Antibody (2469) <br> Blue: DAPI staining
Western blot analysis of IL-21 receptor expression in human HepG2 cell lysate with IL-21 receptor antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

IL-21 Receptor Antibody

CATALOG NUMBER: 2469

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
IL-21 receptor antibody was raised against a synthetic peptide corresponding to amino acids 97 to 111 of human IL-21 receptor precursor.
The immunogen is located within amino acids 80 - 130 of IL-21 Receptor.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
IL-21 receptor antibody can be used for detection of IL-21 receptor by Western blot at 1-2 μg/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Positive Control 2:
Cat. No. 1462 - Rat Lung Tissue Lysate
Predicted Molecular Weight:
Predicted: 60 kDa
Observed: 62 kDa        
Purification:
IL-21 Receptor Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-21 Receptor Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
IL-21 Receptor antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL21R
Additional Names:
IL-21 Receptor Antibody: NILR, CD360, NILR, UNQ3121/PRO10273, Interleukin-21 receptor, Novel interleukin receptor, IL-21 receptor
Protein Accession Number:
NP_068570
Protein Gi Number:
11141869
Ncbi Gene Id Number:
50615
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-21 Receptor Antibody: A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. Both IL-21R and the gammac are necessary for the IL-21 function. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Background Reference 1:
Parrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature. 2000;408(6808):57-63.
Background Reference 2:
Ozaki K, Kikly K, Michalovich D, et al.  Cloning of a type I cytokine receptor most related to the IL-2 receptor β chain. Proc Natl Acad Sci USA. 2000;97(21):11439-44.
Background Reference 3:
Asao H, Okuyama C, Kumaki S, et al. the common γ-chain is an indispensable subunit of the IL-21 receptor complex. J Immunol. 2001;167(1):1-5.
Background Reference 4:
Vosshenrich CA, Di Santo JP. Cytokines: IL-21 joins the γ(c)-dependent network.Curr Biol. 2001;11(5):R175-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2469

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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