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Western blot analysis of IL-22 in HeLa cell lysate with IL-22 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of IL-22 in HeLa cells with IL-22 antibody at 5 μg/mL.
Immunofluorescence of IL-22 in HeLa cells with IL-22 antibody at 20 μg/mL.
Western blot analysis of IL-22 in HeLa cell lysate with IL-22 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

IL-22 Antibody

CATALOG NUMBER: 7593

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
IL-22 antibody was raised against a 17 amino acid peptide near the center of human IL-22 .
The immunogen is located within amino acids 90 - 140 of IL-22.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
IL-22 antibody can be used for detection of IL-22 by Western blot at 0.5 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
IL-22 antibody is human, mouse, and rat reactive. Multiple isoforms of IL-22 are known to exist.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 20 kDa
Observed: 17 kDa
Purification:
IL-22 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-22 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-22 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
IL22
Additional Names:
IL-22 Antibody: TIFa, IL-21, IL-22, ILTIF, IL-TIF, IL-D110, zcyto18, TIFIL-23, ZCYTO18, UNQ3099/PRO10096, Interleukin-22, Cytokine Zcyto18
Protein Accession Number:
NP_065386
Protein Gi Number:
10092625
Ncbi Gene Id Number:
50616
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Interleukin-22 (IL-22) is a cytokine important for the modulation of tissue responses during inflammation (1). Unlike the distantly related IL-10, IL-22 does not inhibit the production of proinflammatory cytokines in monocytes in response to LPS, but it has some inhibitory effects on IL-4 production from Th2 T cells. IL-22 is expressed by both the adaptive arm of the immune system such as CD4 T cell subsets including Th17 cells, as well as by innate lymphocytes such as NK and LTi-like cells (2). IL-22 is highly expressed in several chronic inflammatory conditions, and studies suggest that IL-22 plays both inflammatory and protective roles (3).
Background Reference 1:
Xie MH, Aggarwal S, Ho WH, et al. Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R. J. Biol. Chem. 2000; 275:31335-9.
Background Reference 2:
Wolk K, Kunz S, Witte E, et al. IL-22 increases the innate immunity of tissues. Immunity 2004; 21:241-54.
Background Reference 3:
Wolk K, Witte E, Wallace E, et al. IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis. Eur. J. Immunol. 36:1309-23.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7593

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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