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Western blot analysis of IL-22 receptor expression in human HepG2 cell lysate with IL-22 receptor antibody at (A) 0.5 and (B) 1 μg/ml.
Immunohistochemistry of IL-22R in rat kidney tissue with IL-22R antibody at 5 μg/mL.
Immunofluorescence of IL-22 receptor in HepG2 cells with IL-22 receptor antibody at 10 μg/mL.
Immunocytochemistry of IL-22 receptor in HepG2 cells with IL-22 receptor antibody at 10 μg/mL.
Immunofluorescence of IL-22R in rat kidney tissue with IL-22R antibody at 20 μg/ml.
Western blot analysis of IL-22 receptor expression in human HepG2 cell lysate with IL-22 receptor antibody at (A) 0.5 and (B) 1 μg/ml.
Trial Size

IL-22 Receptor Antibody

CATALOG NUMBER: 2497

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (93%), Bovine: (88%)
Immunogen:
IL-22 receptor antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human IL-22 receptor precursor.
The immunogen is located within the first 50 amino acids of IL-22 Receptor.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Application Note:
IL-22 receptor antibody can be used for detection of IL-22 receptor by Western blot at 0.5-1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.               
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples; Immunocytochemistry in human samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2:
Cat. No. 17-011 - Hep G2 Cell Slide
Predicted Molecular Weight:
Predicted: 63 kDa
Observed: 65 kDa
Purification:
IL-22 Receptor Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-22 Receptor Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-22 Receptor antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL22RA1
Additional Names:
IL-22 Receptor Antibody: IL22R, CRF2-9, IL22R1, IL22R, Interleukin-22 receptor subunit alpha-1, Cytokine receptor class-II member 9, IL-22 receptor subunit alpha-1
Protein Accession Number:
NP_067081
Protein Gi Number:
10719607
Ncbi Gene Id Number:
58985
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-22 Receptor Antibody: A novel cytokine, designated IL-TIF for IL-10 related T cell-derived inducible factor and IL-22, was recently identified. The receptor for IL-22 (IL-22R, also termed CRF2-9 and IL-TIF-R1 chain) is a new member of the class II cytokine receptor family. IL-22R forms a complex with IL-10 receptor beta chain and mediates IL-22 signaling. IL-22 and its receptor activate JAK-STAT signaling pathway. IL22R is expressed in normal liver and kidney and their cell lines HepG2 and TK-10. A soluble form of IL-22 receptor, also termed IL-22 binding protein (IL-22BP) and IL-22 receptor-alpha 2 (IL-22RA2), was identified very recently. IL-22BP prevents binding of IL-22 to the functional cell surface IL-22R complex and neutralizes IL-22 activity. LPS induces IL-22 expression, which indicates the role of IL-22 in inflammatory response.
Background Reference 1:
Dumoutier L, Louahed J, Renauld JC. Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9. J Immunol. 2000;164(4):1814-9.
Background Reference 2:
Dumoutier L, Van Roost E, Colau D, Renauld JC.  Human interleukin-10-related T cell-derived inducible factor: molecular cloning and functional characterization as an hepatocyte-stimulating factor. Proc Natl Acad Sci USA. 2000 ;97(18):10144-9.
Background Reference 3:
Xie MH, Aggarwal S, Ho WH, et al. Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R. J Biol Chem. 2000;275(40):31335-9.
Background Reference 4:
Kotenko SV, Izotova LS, Mirochnitchenko OV, et al. Identification of the functional interleukin-22 (IL-22) receptor complex: J Biol Chem. 2001;276(4):2725-32.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2497

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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