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<strong>Figure 1 Western Blot Validation in Human Raji Cells</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IL-23 3793 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Absence of blocking peptideLane B: Presence of blocking peptide.
<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br>Loading: 20 μg of lysates per lane.Antibodies: IL-23 3793, (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation with Recombinant Protein</strong><br>Loading: 30 ng of human IL-23 recombinant protein.Antibodies: IL-23 3793 (4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunofluorescence Validation of IL-23 in Raji Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed Raji Cells labeling IL-23 with 3793 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
<strong>Figure 5 Immunocytochemistry Validation of IL-23 in Raji Cells</strong><br>Immunocytochemical analysis of Raji cells using anti-IL-23 antibody (3793) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4° C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunofluorescence Validation of IL-23 in Human Pancreas Tissue</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling IL-23 with 3793 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
<strong>Figure 7 Immunohistochemistry Validation of IL-23 in Human Pancreas Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IL-23 antibody (3793) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4° C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 8 Immunohistochemistry Validation of IL-23 in Human Abdominal Aortic Aneurysm (AAA) Tissues (Yan et al., 2019)</strong><br>
Figure 1 Western Blot Validation in Human Raji Cells
Loading: 15 μg of lysates per lane.Antibodies: IL-23 3793 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Absence of blocking peptideLane B: Presence of blocking peptide.
Green Product
Trial Size

IL-23 Antibody

CATALOG NUMBER: 3793

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Guinea pig: (93%), Horse: (93%), Mouse: (71%), Rat: (71%)
Immunogen:
Anti-IL-23 antibody (3793) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of mature human IL-23.
The immunogen is located the last 50 amino acids of IL-23.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Application Note:
WB: 1-4 μg/mL; ICC: 10 μg/mL; IF: 20 μg/mL, IHC: 5 μg/mL.
Antibody validated: Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Positive Control 2:
Cat. No. 11-101 - Human Pancreas Tissue Slide
Positive Control 3:
Cat. No. 17-007 - Raji Cell Slide
Predicted Molecular Weight:
Predicted: 21kD
Observed: 22 kD
Validation:

Recombinant Protein Test (Figure 3): Anit-IL-23 antibodies (3793) detected human IL-23 recombinant protein.

 

Regulated expression validation (Figure 8): IL-23 expression detected by anit-IL-23 antibodies (3793) was abundant in human abdominal aortic aneurysm (AAA) tissues whereas there is no IL-23 expression in normal tissue.

Isoforms:
Human IL-23 has one isoform (189aa, 20.7kD). Mouse IL-23 has one isoform (196aa, 22.1kD) and Rat IL-23 also has one isoform (196aa, 22kD). 3793 can detect human IL-23.
Purification:
IL-23 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
IL-23 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-23 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL23A
Additional Names:
IL-23 Antibody: P19, SGRF, IL-23, IL-23A, IL23P19, UNQ2498/PRO5798, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, IL-23 subunit alpha
Protein Accession Number:
AAH66268
Protein Gi Number:
42542809
Ncbi Gene Id Number:
51561
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-23 Antibody: Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-gamma, IL-12, and TNF-α from activated T cells, and can stimulate macrophages to produce TNF-α and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.
Background Reference 1:
Hunter. Nat. Rev. Immunol. 2005; 5:521-31.
Background Reference 2:
Oppmann et al. Immunity 2000; 13:715-25.
Background Reference 3:
Sheibanie et al. FASEB J. 2004; 18:1318-20.
Background Reference 4:
Pirhonen et al. J. Immunol. 2002; 169:5673-8.
Citation 1:
Yan et al. Interleukin-12 and -23 blockade mitigates elastase-induced abdominal aortic aneurysm. Sci Rep. 2019;9(1):10447.PMID: 31320700

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3793

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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