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Western blot analysis of IL27 using rabbit polyclonal IL27 Antibody (C71) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IL27 gene.
IL-27 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of IL27 using rabbit polyclonal IL27 Antibody (C71) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IL27 gene.

IL-27 Antibody

CATALOG NUMBER: 58-928

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This IL-27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-87 amino acids from the Central region of human IL-27.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL17D
Additional Names:
Interleukin-17D, IL-17D, Interleukin-27, IL-27, IL17D, IL27
Protein Accession Number:
Q8TAD2
Protein Gi Number:
46576614
Ncbi Gene Id Number:
53342
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-27 is a cytokine that shares the sequence similarity with IL17. The treatment of endothelial cells with this cytokine has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF. The increased expression of IL8 induced by this cytokine was found to be NF-kappa B-dependent.
Background Reference 1:
Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
Background Reference 2:
Starnes, T., et al., J. Immunol. 169(2):642-646 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-928

List Size:
400 ul

List Price:

$495.00

Datasheet
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