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Western blot analysis of IL-31 in human skeletal muscle tissue lysate with IL-31 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.
Immunofluorescence of IL-31 in Human Skeletal Muscle cells with IL-31 antibody at 20 μg/mL.
Western blot analysis of IL-31 in human skeletal muscle tissue lysate with IL-31 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.
Green Product
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IL-31 Antibody

CATALOG NUMBER: 3747

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
IL-31 antibody was raised against a 18 amino acid synthetic peptide from near the center of human IL-31.
The immunogen is located within amino acids 60 - 110 of IL-31.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
IL-31 antibody can be used for the detection of IL-31 by Western blot at 2.5 - 10 μg/mL. Despite its predicted size, IL-31 runs at approximately 27 - 30 kDa in SDS-PAGE. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Purification:
IL-31 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
IL-31 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-31 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Il31
Additional Names:
IL-31 Antibody: 1700013B14Rik, Interleukin-31, IL-31
Protein Accession Number:
AAS86448
Protein Gi Number:
46276465
Ncbi Gene Id Number:
386653
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-31 Antibody: Interleukin-31 (IL-31) is a recently discovered T-cell cytokine closely related to IL-6 type cytokines and is preferentially produced by T helper type 2 cells. IL-31 activity is mediated through the ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor. In response to IL-31 binding, these proteins activate the JAK/STAT and the AKT signaling pathways. RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes but are expressed constitutively in epithelial cells. IL-31, when overexpressed in transgenic mice, results in the development of pruritis, alopecia, and skin lesions and in humans may result in atopic dermatitis, suggesting that IL-31 may represent a novel target for antipruritic drug development.
Background Reference 1:
Dillon SR, Sprecher C, Hammond A, et al. Interleukin 31, a cytokine produced by activated T cells, induces dermatitis in mice. Nat. Immunol. 2004; 5:752-60.
Background Reference 2:
Dreuw A, Radtke S, Pflanz S, et al. Characterization of the signaling capacities of the novel gp130-like cytokine receptor. J. Biol. Chem. 2004; 279:36112-20.
Background Reference 3:
Diveu C, Lak-Hal AH, Froger J, et al. Predominant expression of the long isoform of GP130-like (GPL) receptor is required for interleukin-31 signaling. Eur. Cytokine Netw. 2004; 15:291-302.
Background Reference 4:
Bilsborough J, Leung DY, Maurer M, et al. IL-31 is associated with cutaneous lymphocyte antigen-positive skin homing T cells in patients with atopic dermatitis. J. Allergy Clin. Immunol. 2006; 117:418-25.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3747

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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