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Western blot analysis of IL-34 in human brain tissue lysate with IL-34 antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of 1 ng of recombinant human IL-34 with IL-34 antibody at 1 μg/ml.
Western blot analysis of IL-34 in human brain tissue lysate with IL-34 antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

IL-34 Antibody

CATALOG NUMBER: 4779

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
IL-34 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-34.
The immunogen is located within amino acids 30 - 80 of IL-34.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
IL-34 antibody can be used for the detection of IL-34 by Western blot at 0.25 μg/mL. Despite its predicted molecular weight, IL-34 will often run at higher molecular weight in SDS-PAGE.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 26 kDa
Observed: 42-48 kDa
Purification:
IL-34 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-34 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-34 antibody can be stored at 4°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL34
Additional Names:
IL-34 Antibody: IL-34, C16orf77, Interleukin-34, IL-34
Protein Accession Number:
ACD13474
Protein Gi Number:
187610438
Ncbi Gene Id Number:
146433
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-34 Antibody: Interleukin-34 (IL-34) was identified through a functional screening of a library of secreted proteins after transfection of an embryonic kidney cell line with recombinant cDNAs. Flow cytometry and monocyte viability assays demonstrated increased survival and/or proliferation of monocytes induced by IL-34 and specific binding of IL-34 to CD14+ monocytes. IL-34 is a second ligand to the colony stimulating factor (CSF)-1 receptor in addition to CSF1. Like CSF1, IL-34 stimulates phosphorylation of ERK1 and ERK2 in human monocytes and induces differentiation of bone marrow cells into macrophages.
Background Reference 1:
Lin H, Lee E, Hestir K, et al. Discovery of a cytokine and its receptor by functional screening of the extracellular proteome. Science2008; 320:807-11.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4779

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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