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Western blot analysis of IL-36A in MCF7 cell lysate with IL-36A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of IL-36A in human brain tissue with IL-36A antibody at 5 μg/mL.
Immunofluorescence of IL-36A in human brain tissue with IL-36A antibody at 20 μg/mL.
Western blot analysis of IL-36A in MCF7 cell lysate with IL-36A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

IL-36A Antibody

CATALOG NUMBER: 7505

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
IL-36A antibody was raised against an 11 amino acid peptide near the carboxy terminus of human IL-36A.
The immunogen is located within the last 50 amino acids of IL-36A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
IL-36A antibody can be used for detection of IL-36A by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
IL-36A antibody is human specific. IL-36A antibody will not cross-react with IL-36B or IL-36G.
Positive Control 1:
Cat. No. 1219 - MCF7 Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 17 kDa
Observed: 16 kDa
Purification:
IL-36A antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-36A antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-36A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
IL36A
Additional Names:
IL-36A Antibody: FIL1, FIL1E, IL1F6, IL-1F6, IL1(EPSILON), FIL1(EPSILON), IL1E, Interleukin-36 alpha, FIL1 epsilon, IL-1 epsilon
Protein Accession Number:
NP_055255
Protein Gi Number:
7657092
Ncbi Gene Id Number:
27179
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-36A is is a member of the interleukin 1 cytokine family whose gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (1). IL-36A is thought to activate the NF-kappaB pathway through IL-1 receptor family members IL-1RL2 and IL-1RAcP (2). Like the related proteins IL-36B and IL-36G, IL-36A requires post-translational processing for full agonist activity, but the cleavage mechanism is currently unknown (3). The IL-36 cytokines have been suggested to amplify Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells (4).
Background Reference 1:
Smith DE, Renshaw BR, Ketchem RR, et al. Four new members expand the interleukin-1 superfamily. J. Biol. Chem. 2000; 275:1169-75.
Background Reference 2:
Towne JE, Garka KE, Renshaw BR, et al. Interleukin (IL)-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP to activate the pathway leading to NF-kappaB and MAPKs. J. Biol. Chem. 2004; 279:13677-88.
Background Reference 3:
Towne JE, Renshaw BR, Douangpanya J, et al. Interleukin-36 (IL-36) ligands require processing for full agonist agonist (IL-36a, IL-36b, and IL-36g) or antagonist (IL-36Ra) activity. J. Biol. Chem. 2011; 286:42594-602.
Background Reference 4:
Vigne S, Palmer G, Martin P, et al. IL-36 signaling amplifies Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells. Blood 2012; 120:3478-87.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7505

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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