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This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes natural and recombinant human IL-6.<br>The factors listed below were prepared at 10 ng/mL in calibrator diluent and assayed for cross-reactivity. Preparations of the following factors at 10 ng/mL in a mid-range recombinant human IL-6 control were assayed for interference. No significant cross-reactivity or interference was observed.
According to the relevant quality standards, the antibody was sold and placed at 37 degrees for accelerated stability test. After 20 days of destruction, the antibody performance remained stable.
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Recombinant

IL-6 Antibody [2M8M3]

CATALOG NUMBER: 11-094

Clonality:
Recombinant Monoclonal
Tested Applications:
ELISA
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Immunogen:
E. coli-derived human IL-6 protein, amino acids Pro29-Met212
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Human IL-6 Sandwich Immunoassay:

ELISA Capture: Recommended Concentration: 1-4ug/mL, Sample: Human IL-6 Monoclonal Antibody, Rabbit MAb(CAP)

ELISA Detection: Recommended Concentration: 0.03-0.15ug/mL, Sample: Human IL-6 Monoclonal Antibody, Rabbit MAb(DET)

Standard: Recommended Concentration: 0.78-50pg/mL, Sample: Recombinant Human IL6 Protein

Purification:
Affinity Purified, Endotoxin Level: < 1.0 EU/ug of the protein by LAL method.
Clonality:
Recombinant Monoclonal
Isotype:
Rabbit IgG
physical-state:
Liquid
Buffer:
Supplied as a 0.2um filtered solution in PBS, pH 7.4.
Concentration:
1mg/ml
Storage Conditions:
This antibody can be stored at 2°C to -8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
IL6
Additional Names:
CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2
Protein Gi Number:
74739144
Ncbi Gene Id Number:
3569
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilution/concentrations should be determined by the end user. The information provided is a guidline for Product use . The product is for research use only

CATALOG NUMBER:

11-094

List Size:
0.1 mg

List Price:

$626.00

Datasheet
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