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To detect Murine IL-7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-7 (XP-5198Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-7.
To detect Murine IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Murine IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Murine IL-7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine IL-7 (XP-5198Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-7.

IL-7 Antibody

CATALOG NUMBER: XP-5198

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-7 (murine IL-7).
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-7 (0.60 ng/mL), a concentration of 0.012 - 0.02 μg/mL of this antibody is required.
ELISA:
To detect mIL-7 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-7.
Sandwich:
To detect mIL-7 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IL-7 (XP-5198Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-7.
Western Blot:
To detect mIL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-mIL-7 specific antibody was purified by affinity chromatography employing immobilized mIL-7 matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
IL-7 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Il7
Additional Names:
Il-7, hlb368, A630026I06Rik, Il-7, Interleukin-7IL-7
Protein Accession Number:
P10168
Protein Gi Number:
124355
Ncbi Gene Id Number:
16196
User Note:
Centrifuge vial prior to opening.
Background:
Iterleukin 7 is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL-7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL-7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL-7 stimulates the proliferation of CD4+/CD8+ cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5198

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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