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Human IL-7 R alpha, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized ActiveMax Human IL-7, Tag Free at 2 ug/mL (100 uL/well) can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag with a linear range of 4-31 ng/mL (QC tested).
Biotinylated Human TSLP (R127A, R130A), His,Avitag immobilized at 2 ug/mL (100 uL/well) via precoated 5 ug/mL (100 uL/well) of Human TSLP R, Fc Tag, can bind can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. N
Human IL-7 R alpha, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

IL-7 R alpha / CD127 Recombinant Protein

CATALOG NUMBER: 11-538

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Glu 21 - Gly 236
Fusion Tag:
Mouse IgG Fc Tag
Tested Applications:
ELISA, WB
Application Note:
This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 52.0 kDa. The protein migrates as 65-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Predicted Molecular Weight:
52.0 kDa
Purity:
>90% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
Tris with Glycine, Arginine and NaCl, pH7.5
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Additional Names:
IL7Ra,CD127
Background:
Interleukin-7 receptor subunit alpha (IL7Ra) is also known as CD antigen CD127, which belongs to the type I cytokine receptor family and type 4 subfamily. IL7Ra /CD127 contains 1 fibronectin type-III domain. IL7Ra /CD127 is expressed on various cell types, including naive and memory T cells and many others. IL7Ra /CD127 is receptor for interleukin-7 and also acts as a receptor for thymic stromal lymphopoietin (TSLP). The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R.
Background Reference 1:
Noguchi M., et al., 1994, Science 262 (5141): 1877–80.
Background Reference 2:
Kroemer RT., et al., 1997, Protein Eng. 9 (12): 1135–42.
Background Reference 3:
McElroy C.A., et al., 2009, Structure 17:54-65.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-538

List Size:
0.1 mg

List Price:

$540.00

Datasheet
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