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IL-8 Antibody [SB-84-8]

CATALOG NUMBER: XP-5706-M

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
The monoclonal antibody was produced in BALB/c x C57BL/6 F1 mice using recombinant hIL-8 as the immunizing antigen.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-8 when used with our biotinylated antigen affinity purified anti-human IL-8 (XP-5199Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hIL-8 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-8 is 2.0-4.0 ng/lane, under non-reducing conditions.
Purification:
This IgG1 antibody was purified from ascites fluid on a Protein A affinity column.
Clonality:
Monoclonal
Physical State:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
IL-8 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
Ncbi Official Symbol:
IL8
Additional Names:
Interleukin 8, CXCL8, Interleukin-8, C-X-C motif chemokine 8, IL-8, NAF, GCP1, LECT, LUCT, NAP1, GCP-1, LYNAP, MDNCF, MONAP, NAP-1
Protein Accession Number:
P10145
Protein Gi Number:
124359
Ncbi Gene Id Number:
3576
User Note:
Centrifuge vial prior to opening.
Background:
IL-8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL-8 attracts neutrophils, basophils, and T-cells, but not monocytes.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5706-M

List Size:
0.5 mg

List Price:

$376.00

Datasheet
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