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<b> Flow cytometry using the Anti-IL-8RB antibody HC2 ( </b> Paraformaldehyde fixed human peripheral blood monocytes were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of HC2 (24-794- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RB antibody HC2 (</b> Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix™ coverslips stained with the chimeric r version of HC2 (24-794) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-794- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-IL-8RB antibody HC2 ( Paraformaldehyde fixed human peripheral blood monocytes were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of HC2 (24-794- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

IL-8RB Antibody [HC2], Rabbit IgG

CATALOG NUMBER: 24-794

Clonality:
Recombinant Monoclonal
Tested Applications:
Flow
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human
Species:
Rabbit
Original Species Name:
Mouse
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
This antibody was raised by immunizing mice with human IL-8 (interleukin-8) receptor, type B.
Conjugate:
Unconjugated
Tested Applications:
Flow
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
CXCR2
Additional Names:
CXCR-2, CD182, C-X-C chemokine receptor type 2, CXC-R2, CXCR-2, GRO/MGSA receptor, High affinity interleukin-8 receptor B, IL-8R B, IL-8 receptor type 2
Ncbi Gene Id Number:
3579
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-794

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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