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To detect Human IL-9 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-9 (XP-5200Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-9.
To detect Human IL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human IL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human IL-9 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-9 (XP-5200Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-9.

IL-9 Antibody

CATALOG NUMBER: XP-5200

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-9 (human Interleukin-9).
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-9 (0.6 ng/mL), a concentration of 0.15 - 0.025 μg/mL of this antibody is required.
ELISA:
To detect hIL-9 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-9.
Sandwich:
To detect hIL-9 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-9 (XP-5200Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-9.
Western Blot:
To detect hIL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hIL-9 specific antibody was purified by affinity chromatography employing immobilized hIL-9 matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
IL-9 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
IL9
Additional Names:
P40, HP40, IL-9Interleukin-9, Cytokine P40, IL-9
Protein Accession Number:
P15248
Protein Gi Number:
124362
Ncbi Gene Id Number:
3578
User Note:
Centrifuge vial prior to opening.
Background:
IL-9 is a cytokine preferentially produced by T helper type 2 lymphocytes and active on various cell types such as T and B lymphocytes, mast cells and haemopoietic progenitors; but no effect of this cytokine has been reported so far on mononuclear phagocytes. IL-9 stimulates cell proliferation and prevents apoptosis. The IL-9 receptor (IL 9R) belongs to the haemopoietic receptor superfamily and its signal transduction involves mainly the (JAK/STAT) pathway. Some studies have implicated IL-9 as an important cytokine in a number of Th2 cytokine mediated pathologies, in particular asthma

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5200

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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