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<strong>Figure 1 Western Blot Validation in Human Spleen Tissue Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IL-9, 7495 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation with Recombinant Protein</strong><br>Loading: 30 ng of human IL-9 recombinant protein per lane.Antibodies: IL-9, 7495 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 22kD.
<strong>Figure 3 Western Blot Validation in Tissue and Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IL-9, 7495 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunohistochemistry Validation of IL-9 in Human Spleen Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-9 antibody (7495) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 4 Immunohistochemistry Validation of IL-9 in Human Spleen Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-9 antibody (7495) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 Western Blot Validation in Human Spleen Tissue Lysate
Loading: 15 μg of lysates per lane.Antibodies: IL-9, 7495 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

IL-9 Antibody

CATALOG NUMBER: 7495

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Anti-IL-9 antibody (7495) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IL-9.
The immunogen is located within the last 50 amino acids of IL-9.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WB: 1-4 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
IL-9 antibody is human specific. IL-9 antibody is predicted to no cross-react with IL-7.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Positive Control 3:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
Predicted: 16kD
Observed: 22 kD (Post-modification: 4 N-linked glycosylations)
Validation:

Recombinant Protein Test (Figure 2): Anti-IL-9 antibodies (7495) detected human IL-9 recombinant protein at different concentrations.

Isoforms:
Human IL-9 has one isoform (144aa, 16kD). Mouse IL-9 has one isoform (144aa, 16kD) and Rat IL-9 also has one isoform (144aa, 16kD). 7495 can detect human isoform, but cannot detect mouse and rat.
Purification:
IL-9 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-9 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-9 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL9
Additional Names:
IL-9 Antibody : P40, HP40, IL-9, Interleukin-9, Cytokine P40
Protein Accession Number:
NP_000581
Protein Gi Number:
10834980
Ncbi Gene Id Number:
3578
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Interleukin 9 (IL-9) is a cytokine secreted by TH2 lymphocytes that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation and prevents apoptosis (1,2). It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins (3). The IL-9 gene has been identified as a candidate gene for asthma (4). Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness (5).
Background Reference 1:
Renauld et al. Cytokine 1990; 2:9-12.
Background Reference 2:
Tete et al. J. Biol. Homeost. Agents 2012; 26:319-26.
Background Reference 3:
Knoops and Renauld. Growth Factors 2004; 22:207-15.
Background Reference 4:
Nicolaides et al. Proc. Natl. Acad. Sci. USA 1997; 94:13175-80.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7495

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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