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Western blot analysis of extracts of various cell lines, using IL10RA antibody (18-334) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using IL10RA Antibody (18-334) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using IL10RA Antibody (18-334) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using IL10RA Antibody (18-334) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using IL10RA antibody (18-334). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using IL10RA antibody (18-334) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

IL10RA Antibody

CATALOG NUMBER: 18-334

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 22-230 of human IL10RA (NP_001549.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
K-562
Positive Control 2:
MCF7
Positive Control 3:
Mouse brain
Predicted Molecular Weight:
Observed: 72kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IL10RA
Additional Names:
IL10RA, CDW210A, HIL-10R, IL-10R1, IL10R, CD210, CD210a
Ncbi Gene Id Number:
3587
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-334

List Size:
100 uL

List Price:

$436.00

Datasheet
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