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Western blot analysis of extracts of various cell lines, using IL10RB antibody (16-179) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using IL10RB antibody (16-179) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

IL10RB Antibody

CATALOG NUMBER: 16-179

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-220 of human IL10RB (NP_000619.3).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
U-87MG
Positive Control 2:
293T
Positive Control 3:
Jurkat
Positive Control 4:
mouse skeletal muscle
Positive Control 5:
rat heart
Positive Control 6:
rat liver
Predicted Molecular Weight:
Observed: 36kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IL10RB
Additional Names:
CDW210B, CRF2-4, CRFB4, D21S58, D21S66, IL-10R2, interleukin-10 receptor subunit beta, IL-10 receptor subunit beta, IL-10R subunit 2, IL-10R subunit beta, IL-10RB, cytokine receptor class-II CRF2-4, cytokine receptor class-II member 4, cytokine receptor family 2 member 4, cytokine receptor family II, member 4, interleukin 10 receptor, beta, interleukin-10 receptor subunit 2
Ncbi Gene Id Number:
3588
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. This gene and three other interferon receptor genes, IFAR2, IFNAR1, and IFNGR2, form a class II cytokine receptor gene cluster located in a small region on chromosome 21.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-179

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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