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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of IL13 Antibody with NCI-H460 cell line followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
IL13 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

IL13 Antibody

CATALOG NUMBER: 57-235

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This IL13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-146 amino acids from the C-terminal region of human IL13.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
16 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL13
Additional Names:
Interleukin-13, IL-13, IL13, NC30
Protein Accession Number:
P35225
Protein Gi Number:
239938644
Ncbi Gene Id Number:
3596
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an immunoregulatory cytokine producedprimarily by activated Th2 cells. This cytokine is involved inseveral stages of B-cell maturation and differentiation. Itup-regulates CD23 and MHC class II expression, and promotes IgEisotype switching of B cells. This cytokine down-regulatesmacrophage activity, thereby inhibits the production ofpro-inflammatory cytokines and chemokines. This cytokine is foundto be critical to the pathogenesis of allergen-induced asthma butoperates through mechanisms independent of IgE and eosinophils.This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster onchromosome 5q, with this gene particularly close to IL4. [providedby RefSeq].
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Shi, X., et al. Anat Rec (Hoboken) 293(9):1470-1476(2010)
Background Reference 3:
Beckers, M.M., et al. Eur. J. Intern. Med. 21(4):289-292(2010)
Background Reference 4:
Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010) In press :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-235

List Size:
400 ul

List Price:

$495.00

Datasheet
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