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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
Western blot analysis of IL17B using rabbit polyclonal IL17B Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IL17B gene.
IL17B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of IL17B Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

IL17B Antibody

CATALOG NUMBER: 56-167

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This IL17B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-66 amino acids from the Central region of human IL17B.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
20 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL17B
Additional Names:
Interleukin-17B, IL-17B, Cytokine Zcyto7, Interleukin-20, IL-20, Neuronal interleukin-17-related factor, IL17B, IL20, NIRF, ZCYTO7
Protein Accession Number:
Q9UHF5
Protein Gi Number:
20143877
Ncbi Gene Id Number:
27190
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a T cell-derivedcytokine that shares sequence similarity with IL17. This cytokinewas reported to stimulate the release of TNF alpha (TNF) and IL1beta (IL1B) from a monocytic cell line. Immunohistochemicalanalysis of several nerve tissues indicated that this cytokine isprimarily localized to neuronal cell bodies.
Background Reference 1:
Koskinen, L.L., et al. Tissue Antigens 74(5):408-416(2009)
Background Reference 2:
Wang, X., et al. Ann. Otol. Rhinol. Laryngol. 115(6):450-456(2006)
Background Reference 3:
Ryan, A.W., et al. Tissue Antigens 65(2):150-155(2005)
Background Reference 4:
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-167

List Size:
400 ul

List Price:

$495.00

Datasheet
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