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Western Blot at 1:2000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:2000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.

IL1R Antibody

CATALOG NUMBER: 62-259

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This IL1R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-503 amino acids from the C-terminal region of human IL1R.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
65 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL1R1
Additional Names:
Interleukin-1 receptor type 1, IL-1R-1, IL-1RT-1, IL-1RT1, CD121 antigen-like family member A, Interleukin-1 receptor alpha, IL-1R-alpha, Interleukin-1 receptor type I, p80, CD121a, Interleukin-1 receptor type 1, membrane form, mIL-1R1, mIL-1RI, Interleukin-1 receptor type 1, soluble form, sIL-1R1, sIL-1RI, IL1R1, IL1R, IL1RA, IL1RT1
Protein Accession Number:
P14778
Protein Gi Number:
124308
Ncbi Gene Id Number:
3554
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL1R is a member of the interleukin 1 receptor family. An experiment with transient gene expression demonstrated that this receptor was incapable of binding to interleukin 1 alpha and interleukin 1 beta with high affinity. IL1R is a receptor for interleukin 1 family member 9 (IL1F9). Binding to the agonist leads to the activation of NF-kappa-B. The gene for this protein and four other interleukin 1 receptor family genes, including interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 1 (IL1RL1), and interleukin 18 receptor 1 (IL18R1), form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
Background Reference 1:
Debets, R., et al., J. Immunol. 167(3):1440-1446 (2001).
Background Reference 2:
Dale, M., et al., Genomics 57(1):177-179 (1999).
Background Reference 3:
Torigoe, K., et al., J. Biol. Chem. 272(41):25737-25742 (1997).
Background Reference 4:
Lovenberg, T.W., et al., J. Neuroimmunol. 70(2):113-122 (1996).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-259

List Size:
400 ul

List Price:

$495.00

Datasheet
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